Optimization of the Procedure for a Ligninolytic Enzymes Isolation from the White-rot Fungi

The white-rot fungi produce wide range of extracellular enzymes (especially ligninolytic enzymes) and they can degrade complicated and difficult degradable compounds. An immobilization of enzymes causes their stability, reusability and cheapness, so they could be effectively used in biotechnologies and other branches. In this work we focused on the optimization of isolation procedure namely precipitation the enzymes, which will be used for immobilization in the next step. 75% ammonium sulphate was used to salting the enzymes and experimentally set the optimum pH for two species of white-rot fungi – Trametes versicolor and Fomes fomentarius. Our results show that the optimum pH for precipitation by ammonium sulphate is different for used species. Consequently, pH optimum for Trametes versicolor was 6, for Fomes fomentarius was 8.